The role of Importin-7 (IPO7) during activity-dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons
Chin, Pei Wern
Date of Issue2017-07-31
School of Biological Sciences
Importin-7, a member of the importin-beta superfamily of nuclear adaptor proteins, is thought to shuttle activated MAPK into the nucleus to induce transcription-dependent encoding of long-term memories in Drosophila. However, little is known about the function of importin-7, its cellular distribution, mechanisms of action and the additional cargo proteins it binds to. This project aims to characterize importin-7 in rodent hippocampal neurons with the ultimate goal of deciphering its function during learning and memory. Our initial findings indicate that importin-7 is localized at the cytosol, dendrites and spines in mature hippocampal neurons. We found that cytosolic importin- 7 undergoes activity-dependent translocation into the nucleus upon chemical long-term potentiation in acute whole brain slices. To identify cargo proteins that bind to importin-7 in the brain, we performed co-immunoprecipitation studies for importin-7 and analyzed affinity-purified proteins via mass spectrometry, but results are currently pending. Overall, our initial characterization studies hint that importin-7 could be involved in the activity-dependent transport of synaptic proteins into the nucleus during stimuli associated with transcription-dependent long-term plasticity.
DRNTU::Science::Biological sciences::Molecular biology
Final Year Project (FYP)
Nanyang Technological University