Tissue imaging using nonlinear laser scanning microscope
Seeto, David Weng Hwee
Date of Issue2017-05-24
School of Chemical and Biomedical Engineering
Linear optics have been commonly used in microscopy around since invention. However, traditional optical imaging approaches based on linear optics have certain limitations such as out-of-focus fluorescence and limited penetration depth for bio-imaging. All these limitations can be avoided through various novel non-linear optical imaging methods, which are enabled by recent advance on ultrafast laser technology. This report aims to give an overview of how tissues can be imaged using non-linear optics approaches such as two photon excitation fluorescence and second harmonic generation. Within this report includes the principles of light, how light is perceived, the basic setup of a microscope, how fluorescence can be generated, basic setup of a fluorescence microscope, the improvisation made to fluorescent microscopes, the principle of laser operations, non-linear optics, formation of digital image, setup of a laser scanning microscope and how to align a laser system for ideal resolution.
DRNTU::Science::Physics::Optics and light
Final Year Project (FYP)
Nanyang Technological University