View Item 
      •   Home
      • 1. Schools
      • College of Science
      • School of Physical and Mathematical Sciences (SPMS)
      • SPMS Theses
      • View Item
      •   Home
      • 1. Schools
      • College of Science
      • School of Physical and Mathematical Sciences (SPMS)
      • SPMS Theses
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.
      Subject Lookup

      Browse

      All of DR-NTUCommunities & CollectionsTitlesAuthorsBy DateSubjectsThis CollectionTitlesAuthorsBy DateSubjects

      My Account

      Login

      Statistics

      Most Popular ItemsStatistics by Country/RegionMost Popular Authors

      About DR-NTU

      Site-specific localized labeling for efficient bio-sensing of drug resistant bacterial strains

      Thumbnail
      Kock Si Min_Thesis.pdf (4.486Mb)
      Author
      Kock, Si Min
      Date of Issue
      2016
      School
      School of Physical and Mathematical Sciences
      Abstract
      β-lactam antibiotics are a crucial class of drugs widely used to target bacterial infections. However, the frequent use and misuse of β-lactam antibiotics has resulted in the emergence of antibiotic resistance – a severe threat to global health – which is present dominantly through the production of β-lactamase. In response, our group sought to develop optical imaging probes that would enable the rapid and selective intracellular identification and imaging of β-lactamase activity in real-time. We hope the optical imaging probes would serve as a tool for diagnostic microbiology – to screen and identify resistant bacterial strains – to administer controlled antibiotic therapy and to enable more in-depth research in antibiotic resistance mechanisms. In our study, we designed two optical imaging probes: (1) Upconversion nanoparticles (UCNP)-based optical probe (2) Metabolic optical probe for covalent labeling of the bacterial peptidoglycan. The UCNP-based optical probe was observed to have achieved a 150-fold fluorescence enhancement when cleaved with a Class A β-lactamase. The UCNP-based optical probe has also displayed selectivity for resistant bacterial strains and has viable in vitro activity in preliminary mammalian cell fluorescence imaging studies conducted. The metabolic optical imaging probe was observed to have achieved a 200-fold fluorescence enhancement when cleaved with a Class C β-lactamase. The metabolic optical probe has also shown specificity for resistant bacterial strains, displaying greater selectivity for Class C β-lactamase-producing bacterial strains over Class A β-lactamase-producing bacterial strains in fluorescence bacterial imaging studies conducted.
      Subject
      DRNTU::Science::Chemistry::Biochemistry
      Type
      Thesis
      Collections
      • SPMS Theses

      Show full item record


      NTU Library, Nanyang Avenue, Singapore 639798 © 2011 Nanyang Technological University. All rights reserved.
      DSpace software copyright © 2002-2015  DuraSpace
      Contact Us | Send Feedback
      Share |    
      Theme by 
      Atmire NV
       

       


      NTU Library, Nanyang Avenue, Singapore 639798 © 2011 Nanyang Technological University. All rights reserved.
      DSpace software copyright © 2002-2015  DuraSpace
      Contact Us | Send Feedback
      Share |    
      Theme by 
      Atmire NV
       

       

      DCSIMG