dc.contributor.authorLim, Eric Jit-Kai
dc.date.accessioned2016-11-22T02:54:38Z
dc.date.available2016-11-22T02:54:38Z
dc.date.issued2016
dc.identifier.urihttp://hdl.handle.net/10356/69194
dc.description.abstractDengue is a serious disease with a particularly high transmission rate in the tropics. There is currently only one WHO-approved vaccine, but it has limitations. A key therapeutic target on the dengue virus is the envelope glycoprotein (E protein) dimer which coats the outer surface. A new type of antibody has been discovered that binds to the E-dimer dependent epitope (EDE) which involves both monomers within the same epitope. However, the exact mechanism of action is unknown. It is possible that it locks the monomers to prevent subsequent acid-induced dissociation and trimerisation that are required for viral fusion with the cell. A computer simulation study was designed to investigate the mechanism of the EDE2 B7 antibody in complex with the soluble ectodomain of the E dimer. Metadynamics was used in molecular dynamics simulations of the E protein dimer with and without the antibody in neutral and acidic conditions. Bias potentials were applied on the collective variables of inter-dimer contacts, inter-dimer distance and root-mean square deviation during the simulation to enhance conformational sampling. Acidic conditions were found to destabilise the dimer while the presence of the antibody maintained the binding between the monomers, thus highlighting the therapeutic potential of this antibody.en_US
dc.format.extent32 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Science::Biological sciencesen_US
dc.titleInvestigating the structure of the DENV-2 envelope protein in complex with the EDE2 B7 antibody using metadynamics simulationsen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorLu Lanyuanen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US


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