Studying the signalling pathways involved in ANGII-AT2 receptor interactions on beige adipocytes formation
Ho, Ingrid Mei Ying
Date of Issue2016
School of Chemical and Biomedical Engineering
Extensive studies on the adipokines secreted by WAT have shown the potential that they have on the browning of WAT to produce beige adipocytes that have functions liken to BAT with increased basal activity and energy expenditure. The discovery of AngII-AT2 interaction on WAT which drives the PI3k/Akt and AMPK signaling pathways has shown to be able to promote browning in mouse white adipocytes. The extent of its effect would be studied in this paper whereby mouse WAT would be incubated with AngII-AT2 and ZD7155 and specific blockers for each of the pathways to understand the interaction between each of the proteins and measured by the expression of proteins UCP1, CITED1 and aP2. Results are promising with confirmation of AngII-AT2 activating the two pathways and increasing browning of WAT by a large extent. As adipokines tend to be overly expressed during obesity, it could bring about a positive feedback loop that allows for the browning of WAT and to minimize the health complications that WAT bring about, tackling the problem of obesity.
Final Year Project (FYP)
Nanyang Technological University