Decellularisation system For porcine esophagus
Leong, Kenneth Jian Wei
Date of Issue2016-05-25
School of Mechanical and Aerospace Engineering
Tissue engineering construction is greatly influenced by the use of different scaffold material. The decellularised esophagus is one of the more promising material for esophageal tissue engineering. The aim of this project is to identify the different effects of decellularisation on the porcine esophagus by using different concentrations of Sodium Dodecyl Sulphate (SDS). SDS is an iconic detergent, together with Sodium Deoxycholate and Triton X-200, are one of the most commonly used solutions for decellularisation. SDS has been a highly efficient option to remove cytoplasmic proteins and nuclear remnants. However, it may compromise the extracellular matrix or simply put the scaffolding structure. The process is that porcine esophagus are decellularised by using different concentrations of 1%, 3% and 5% SDS. The different samples of differing concentrations are put through a decellularisation process across a period of time before it will be put through examination. After the decellularisation process, the sample is then put through histology, SEM and tensile testing. The results have shown that decellularisation using SDS has proven to be effective in removing most the cells and DNA. SEM has revealed a well preserved scaffold which could definitely be used for tissue engineering. However, concentrations play an important role as the samples of the higher concentrations of SDS has shown that their collagenous fibres are compromised. Simply put, higher concentrations of SDS damages the scaffolding structure of the esophagus.
Final Year Project (FYP)
Nanyang Technological University