The role of Pf4 filamentous bacteriophage repressor C in immunity to reinfection
Goh, Yu Fen
Date of Issue2016
School of Biological Sciences
Pseudomonas aeruginosa is a widespread opportunistic pathogen in many chronic biofilm-based infections. The Pf4 filamentous bacteriophage in PAO1 was previously shown to play an important role during biofilm formation. In particular, the superinfective morphotype was found to be critical for cell death, biofilm dispersal, and variant formation. A putative repressor c homolog, located within the intergenic region between PA0716 and PA0717 in the Pf4 phage genome, is suspected to play a role in protection of the lysogenised bacterial host from Pf4 reinfection. Mutations in this repressor c gene have also been observed in superinfective Pf4. In this study, a plasmid encoding repressor c was constructed to perform in vitro immunity assays to test its role in immunity. Binding assays were also carried out to determine repressor C binding to its upstream putative promoter region. It was observed that repressor C was able to bind to the putative promoter and prevent plaque formation in the immunity assay. Furthermore, the protein failed to bind to a promoter carrying two single nucleotide polymorphisms found in the superinfective phage. The findings of this study provide evidence that the putative repressor c and its promoter region confer immunity to PAO1 against reinfections by Pf4 phage.
Final Year Project (FYP)
Nanyang Technological University
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