Investigation on the cardio-protective properties of Acetyltanshinone IIA against Sunitinib induced cardio-toxicity and their anti-angiogenesis properties
Sim, Jiang Wei
Date of Issue2016-05-24
School of Chemical and Biomedical Engineering
This paper aims to address the cardio-protective effects of Acetyltanshinone IIA (ATA) against Sunitinib induced cardio-toxicity through its effect on the heart rate and the size of pericardium in Zebrafish embryos. At the same time, effects of Sunitinib and ATA as an anti-angiogenesis agent will also be evaluated through Cell Viability and Wound Healing Assays. To evaluate the cardio-toxic and cardio-protective properties of Sunitinib and ATA respectively, Zebrafish embryos are exposed to different concentrations of Sunitinib and ATA 8 hours after they are laid. The heart rate and the pericardium size of the Zebrafish is then evaluated across different time points. To evaluate the anti-angiogenesis properties of Sunitinib and ATA, HUVEC-C3 and H9C3 cells are seeded on 12-well cell culture plates and a wound is created to investigate the rate which the wound closes when exposed to different concentration of Sunitinib and ATA. The same cells are also seeded on a 96-well cell culture plate and the cellular activity is determined through the amount of light absorbance in at MTT Assay. The results of the Zebrafish experiment shows that ATA is able to counter the cardio-toxic effects of Sunitinib through its cardio-protective effects by inducing the activation of AMPK pathways, a molecule that is inhibited by Sunitinib while acting as a RTK inhibitor in its action against cancer cells. At the same time, Sunitinib is a more effective anti-angiogenesis agent as compared to ATA based on the results obtained from the Cell Viability and Wound Healing Assays.
Final Year Project (FYP)
Nanyang Technological University