Development of UV-crosslinkable hybrid 3D hydrogels
Date of Issue2016-04-12
School of Materials Science and Engineering
The project aims to develop UV-crosslinkable hybrid 3D hydrogel with tailorable physical and chemical properties for future cell culture studies in the field of tissue engineering. A hydrogel system were designed by incorporating FN910 protein into a PEG precursor. The protein were expressed and purified successfully, and were subsequently crosslinked into the PEG via UV-initiated thiol-ene click chemistry. The resulting hydrogel were characterized by varying the ratio of the protein and the PEG. The hydrogel system was shown to be tunable into a wide range of stiffness, demonstrating their promise in imitating natural body tissues, while being independent from biochemical cues.
Final Year Project (FYP)
Nanyang Technological University