Biochemical and biophysical characterization of human 15LOX-2 and protein : protein interactions study of sphingosine kinase 1
Date of Issue2015
School of Biological Sciences
Lipid metabolism involved in the regulation of many cellular processes such as cell growth, inflammation, apoptosis, and more are controlled by multiple lipid signaling pathways. I have been working on three of these pathways, namely phosphoinositide signaling, arachidonic acid metabolism, and sphingolipids signaling pathway. De-regulation of these pathways occur when the signaling molecules homeostasis are disturbed, which in turns result in pathogenesis of human diseases. In this thesis, I will focus on the h15LOX-2 in the arachidonic pathway and sphingosine kinase 1 in sphingolipids signaling pathway. By studying the biophysical characteristic of these two enzymes, I hope to understand the mechanisms in disease progression and to provide new leads in drug development.