Viability nanosensors to evaluate cell interaction with novel nanomaterials
Goh, Candice Yun Si
Date of Issue2015
School of Chemical and Biomedical Engineering
The engineering of cells using nanoparticles is a promising approach for integration free labelled cells as well as to determine the viability of the implanted cells for treatments. Particularly in the medical field, it is essential to understand the properties of nanoparticles and any possible effects on the human body before clinical use. Despite the potential nanoparticles display, limited researches are done on the toxicity of nanoparticles and little is known about the toxicity of different nanoparticles due to varying experimental controls in the studies that have been done. Therefore, it is difficult to determining whether the cytotoxicity observed is physiologically relevant. In this study, characterization of calcein-AM loaded PLGA microparticles coated with poly-l-lysine illustrates enhanced intracellular internalization. Furthermore, cytotoxicity of various and novel nanoparticles are investigated to determine an easy and safe method to quantify the viability of nanosensors. Thereafter, in vitro experiment of novel nanoparticles demonstrated that they can non-invasively determine cell viability. Hence, the study of cytotoxicity of various and novel nanoparticles is useful for identifying safe and easy methods to engineer cells for therapeutic treatments.
Final Year Project (FYP)
Nanyang Technological University