Biaxial bioreactor based generation of tissue engineered bone grafts
Lee, Boon Tat
Date of Issue2015
School of Chemical and Biomedical Engineering
Bone tissue engineering (BTE) has been proposed as a promising strategy to develop off-the-shelf tissue engineered bone grafts (TEBG), to treat fractures and overcome the drawbacks of conventional treatments. TEBG currently relies on the integration of three disciplines, scaffold, stem cell and dynamic culture technologies such as bioreactors to achieve clinical utility. Bi-axial bioreactor has shown to be efficient in achieving cellularization as compared to other conventional bioreactor systems. With configurational modifications, the biaxial bioreactor has to be operated under partial bi-axial rotation conditions (sub-optimal capacity). This study aims to determine if the partial bi-axial rotation will still show desirable cellular proliferation, homogenous distribution and osteogenesis. Proliferation, mineralization and cell viability was studied after 14 days of partial bi-axial rotation. Results gathered showed increased proliferation, with total double-stranded DNA content for the partial bi-axial bioreactor culture on day 14 (1.4×) more than static culture. Also, observation through FDA/PI showed increased cell viability and homogenous cellular distribution.
Final Year Project (FYP)
Nanyang Technological University