dc.contributor.authorStephanie
dc.date.accessioned2015-05-29T08:30:29Z
dc.date.available2015-05-29T08:30:29Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.identifier.urihttp://hdl.handle.net/10356/64732
dc.description.abstractEmbryogenesis is a tightly controlled process which involves a plethora of transcription factors to ensure proper development. One group of genes involved in embryogenesis is Hox genes which encode for transcription factors and help in anterior-posterior patterning at post-gastrulation time. However, there are also reports on Hox genes such as hoxd4a acting at pre-gastrulation or peri-gastrulation, implicating their roles in other processes such as haematopoiesis or neural patterning. This project aims to study the expression of a total of eight hox genes in early zebrafish embryo: seven hox genes by whole-mount in situ hybridisation (WISH) and eight hox genes by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The results showed expression hoxb6b and hoxb7a at early times, whilst hoxb3a, hoxa4a and hoxb4a are poorly or not expressed at early times and the results for hoxd3a and hoxc4a are inconclusive. The qRT-PCR result for hoxd4a was also inconclusive. A repeat of WISH and qRT-PCR using different primers and probes as well as increased sample size will help to confirm these results. Nonetheless, the results are useful in building up knowledge about Hox genes expression at early times and setting up foundations for future works.en_US
dc.format.extent43 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Science::Biological sciences::Zoology::Embryologyen_US
dc.subjectDRNTU::Science::Biological sciences::Zoology::Vertebratesen_US
dc.subjectDRNTU::Science::Biological sciences::Geneticsen_US
dc.titleHox gene expression in early zebrafish embryoen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorMark Stephen Featherstoneen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US


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