dc.contributor.authorLiew, Andy Wen Loong
dc.date.accessioned2014-06-09T03:54:22Z
dc.date.available2014-06-09T03:54:22Z
dc.date.copyright2014en_US
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10356/61316
dc.description.abstractTissue engineering (TE) is a field of study which relies on engineering principles and life sciences in order to develop biocompatible structures (scaffolds) for the replacement, restoration, improvement, or assisted growth of human tissue. Implantable scaffolds provide a promising and alternative method to TE. Cells from the host can be cultured in-vitro onto the scaffolds and after sufficient proliferation and cell differentiation, the scaffold can then be implanted into bone defects or areas with damaged tissue allowing the target area to repair itself naturally. A common material used for these scaffolds is Hydroxyapatite (HA) because of its bioactivity and biocompatibility. One downside of pure HA is its weak mechanical properties, which limits its applications in hard tissue prosthetics. Alumina on the other hand is bio-inert, but it possesses far superior mechanical strength. This report investigates the compositional and bioactive properties of HA coated, 3D printed Alumina hybrid scaffolds for application in bone tissue engineering. Ceramic scaffolds made of bio-inert Alumina were fabricated via 3D printing. Scaffolds then underwent several processes of sintering, vacuum infiltration, and dip-coating with HA. TGA test was performed on the printed green part to evaluate the samples’ chemical compositions just after printing. Samples were also submerged in Simulated Bodily Fluid (SBF) for various amounts of time, allowing an Apatite layer to form naturally on the surface and within the pores of the scaffolds. FTIR and EDX was performed on these submerged samples to quantify and compare the elements found on each sample. SEM was used to visually quantify the amount of Apatite forming on each sample, as well as to evaluate the pore sizes present in the scaffolds. Finally, MSCs were culture onto SBF submerged samples to test for cell viability using the PrestoBlue® reagent.en_US
dc.format.extent47 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Engineering::Bioengineeringen_US
dc.titleCharacterization of 3d printed substrates for cell cultureen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.description.degreeMECHANICAL ENGINEERINGen_US
dc.contributor.organizationSINGAPORE INSTITUTE OF MANUFACTURING TECHNOLOGYen_US
dc.contributor.supervisor2Zhang, Yileien_US


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