dc.contributor.authorPhang, William Jak Soon
dc.date.accessioned2014-06-05T06:50:06Z
dc.date.available2014-06-05T06:50:06Z
dc.date.copyright2014en_US
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10356/61139
dc.description.abstractBiofilm mode of growth offers multiple benefits in the biodegradation of toxic xenobiotics. This project aims to enhance the biofilm formation capability of Comamonas testosteroni WDL7 through expressing a protein YedQ that synthesizes c-di-GMP, a key signaling molecule in biofilm formation. A C. testosteroni mutant containing pYedQ2 plasmid was constructed and the effects of c-di-GMP overproduction on phenotype, biofilm formation, and 3-chloroaniline degradation were investigated. The mutant exhibited smaller cell colony and enhanced exopolysaccharide production and inhibited swimming motility. A significant increase in biofilm formation was observed in comparison with wild type. The growth and biodegradation profile of C. testosteroni WDL7 (pYedQ2) under low surface/volume ratio (culture flasks) could be described by the Monod model. In high surface to volume ratio situation, the mutant showed less planktonic growth but a significantly higher contribution of biofilms in 3-chloroaniline degradation. Future experiments will focus on better quantification of biofilm biomass, verification of degradation efficiency and gene expression due to elevated c-di-GMP level.en_US
dc.format.extent41 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Engineering::Environmental engineering::Water treatmenten_US
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Bacteriaen_US
dc.titleCyclic-di-GMP signalling in 3-chloroaniline biodegradation by comamonas testosteronien_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Civil and Environmental Engineeringen_US
dc.description.degreeENVIRONMENTAL ENGINEERINGen_US
dc.contributor.organizationSingapore Centre on Environmental Life Sciences Engineeringen_US
dc.contributor.supervisor2Cao Bin (CEE)en_US


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