Drug-herb interactions on pseudomonas aeruginosa
Date of Issue2008
School of Mechanical and Aerospace Engineering
BioMedical Engineering Research Centre
The aim of this study was to determine if synergism was observed for various combinations of antibiotics with the phytomedicines against American Type Culture Collection (ATCC) strain of P.aeruginosa. The minimum inhibitory concentration (MIC) of these antibiotics and phytomedicines were determined by microdilution method. The results from both the checkerboard and killing curve methodologies by microdilution method were compared. The results of this study indicate that against P.aeruginosa, synergism is observed in combination with some western antibiotics and phytomedicines. Synergism appears to be maintained even at very high MICs with drug concentrations within achievable therapeutic ranges. With current definitions of synergism there was absolute correlation between the results obtained by the checkerboard and killing curve methodologies, with the fractional inhibitory concentration indices and killing curves resulting in synergism. No antagonism was observed.