Design and construction of optical tweezers : single-molecule study of DNA mechanical properties.
Lim, Ci Ji.
Date of Issue2009
School of Physical and Mathematical Sciences
Optical tweezer is an integral tool in the research frontiers of science and engineering. In this FYP, a dual beam optical tweezers with single molecule detection capability is designed and constructed. The process of design and construction is discussed in this thesis. The constructed optical tweezer has a laser power throughput of 66% and maximum optical trapping force of 50 pN. Fundamental experiments were done to demonstrate the optical tweezer applicability. 3 um polystyrene beads coated with fluorescent-tagged complementary ssDNA were optically manipulated to form stable heterogenous pair. Preliminary optical pulling of a 1 um dsDNA tether was also done using the constructed optical tweezers.
Final Year Project (FYP)