Surface modification of grooves on embedded fiber optic surface to faciliate immobilization of antibodies
Chan, Chin Yong.
Date of Issue2009
School of Electrical and Electronic Engineering
In the recent years, many researches had been done on point-of-care bioassay system, people has been finding ways to improve the system in terms of its size, cost and even the capability of the system. It is the speed at which the result of the sample can be collected that induce people to research on this system whereby improvements had been made such that the results can be out at a speed of 10 times faster than the last technology whereby time taken is reduced from hours to minutes. Not only is people interested in the speed, people are also interested in improving its size, its cost of building and also the materials used. In this study, we would be looking into the type of material that can be use to build the micro-fluidic chip. We would test out the material that we have selected to see if it fits our requirement in terms of chemical aspect. The reason why there is a need to look for new material because the current material which is polystyrene is hard to fabricate and brittle to handle. We had selected PMMA to be our replacement for polystyrene because it is easy to fabricate and save cost; we are going to find out whether is there any positive bonding between the antibody and our substrate in this report. In this report, we had proven that there are positive bonding between the antibody and our substrate using series of different chemicals which are mostly easily available in labs in order to minimize the cost. Atomic Force Microscope (AFM) machine was used to scan our sample for the final results. Images of the results will be shown the section of result and discussion in proving of the positive bonding.
Final Year Project (FYP)
Nanyang Technological University