Association study of 3 single-nucleotide polymorphisms of the serum amyloid A1 gene with coronary artery disease in a Singaporean Chinese population.

Abstract

Coronary artery disease (CAD) is a complex inflammatory disease that is a leading cause of death in developed countries. The serum amyloid A (SAA) protein family, which has been found to play multiple roles in lipid metabolism and immune regulation, is thought to contribute to CAD development. Previous work in this laboratory has shown that an exonic non-synonymous single nucleotide polymorphism (SNP) in the human SAA1 gene increases CAD risk in a Singaporean Chinese population. Promoter and intronic SNPs could also affect the development of CAD by modulating basal SAA levels. Hence, in this study, a case-control analysis of the association between three non-exonic SNPs of human SAA1 (-2G>A, -250G>A and IVS3+28A>G) and the occurrence of CAD was performed for a Singaporean Chinese population. For each SNP, approximately 350 each of cases and controls were genotyped using allele-specific PCR. The 95% confidence intervals for the allelic odds ratios were 0.68-1.95 (-2G>A), 0.35-1.73 (-250G>A) and 0.71-1.15 (IVS3+28A>G). None of the SNPs were found to contribute significantly to CAD risk in the population.